Spectrophotometric Method for the Determination of Cefotaxime Sodiumand Cefoperazone Sodium in Pure and Pharmaceutical Dosage Forms

Accuratespectrophotometric method was developed for the estimation of cefotaxime sodium (I) andcefoperazone sodium (II) in both pure and pharmaceutical dosage forms. The method is based on the reaction of the amino groups of the cited drugs with ninhydrin reagent producing a colored product which absorbs maximally at 568 nm. Beer's law was obeyed in the concentration range of (10-90) and (60-350) μg.mL-1 for drug (I) and (II) respectively .The correlation coefficient (r2),molar absorptivity (ε), sandell sensitivity, detection (LOD) and quantitation limits (LOQ) for the studied drugs were calculated. The proposed method was successfullyapplied for the determination of certain pharmaceutical dosage forms containing the studied drugs.